Mouse Anti IL-6 Monoclonal Antibody In Vivo Antibody - Low Endotoxin

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SKU:
IVMB0363
Product Type:
In Vivo Monoclonal Antibody
Clone:
MP5-20F3
Protein:
IL-6
Isotype:
IgG1 k
Reactivity:
Mouse
Synonyms:
Interleukin-6
Synonyms:
IL6
Synonyms:
Anti-Mouse IL6
Synonyms:
Anti-Mouse IL-6
Synonyms:
BCSF
Research Area:
Immunology
Research Area:
Innate Immunity
Endotoxin Level:
Low Endotoxin
Host Species:
Rat
Applications:
ELISA Cap
Applications:
ELISPOT
Applications:
FA
Applications:
In Vivo
Applications:
N

Description

Product Name:Mouse Anti IL-6 Monoclonal Antibody In Vivo Antibody - Low Endotoxin
Product Code:IVMB0363
Size:0.5 mg, 1 mg, 5 mg
Clone:MP5-20F3
Protein:IL-6
Product Type:Monoclonal Antibody
Synonyms:Interleukin-6, IL6, Anti-Mouse IL6, Anti-Mouse IL-6, BCSF
Isotype:IgG1 k
Reactivity:Mouse
Applications:ELISA Cap, ELISPOT, FA, In Vivo, N
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:<0.1 EU/µg as determined by the LAL method
Purity:>95% by SDS-PAGE and HPLC
Product Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:ELISA Cap, ELISPOT, FA, In Vivo, N
Reactivity:Mouse
Host Species:Rat
Specificity:Clone MP5-20F3 recognizes an epitope on mouse IL-6.
Concentration:1.0 mg/ml
Endotoxin Level:<0.1 EU/µg as determined by the LAL method
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:>95% by SDS-PAGE and HPLC
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:This Low Endotoxin Functional Formulation conjugate is stable when stored at 2-8°C. Do not freeze. For long term storage, aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IL-6 is a pleotropic 26 kD protein that can act as both a pro-inflammatory cytokine and an anti-inflammatory myokine, a form of cytokine produced in muscle cells that participates in tissue regeneration and repair, maintenance of healthy bodily functioning, and homeostasis within the immune system. IL-6 plays a part in the immune, endocrine, nervous, and hematopoietic systems, in addition to bone metabolism, regulation of blood pressure and inflammation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. Furthermore, IL-6 is an important mediator of fever and of the acute phase response which is the body's rapid attempt to restore homeostasis after tissue injury, infection, neoplastic growth, or immunological disturbance. In addition, IL-6 can be released into circulation in response to various stimuli including PAMPs (pathogen-associated molecular patterns) and cortisol, a hormone produced by the human body under psychologically stressful conditions. In its role as an anti-inflammatory myokine, IL-6 precedes the appearance of other cytokines in the circulation, is notably elevated with exercise, and is mediated by both its inhibitory effects on TNF-α and IL-1, and activation of IL-1ra and IL-10. IL-6 signals through a cell-surface type I cytokine receptor complex formed by the binding of IL-6 to IL-6R, forming a binary complex, which in turn combines with GP130 to transduce extracellular signaling by the activation STAT3. Hence, it is thought that blocking the interaction between IL-6 and GP130 may have therapeutic potential via the inhibition of the IL-6/GP130/STAT3 signaling pathway. Moreover, IL-6 initiates the inflammatory and auto-immune processes in many diseases such as diabetes, atherosclerosis, depression, Alzheimer's disease, rheumatoid arthritis, cancer, and various others. Thus, there is an interest in the therapeutic potential of anti-IL-6 mAbs.

Protein:IL-6
Research Area:Immunology, Innate Immunity
Shipping:2-8°C
PubMed:IL-6
NCBI Gene Bank ID:16193
UniProt:CD275
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