Enzymes Recombinant Proteins

Mouse ALPL Recombinant Protein (RPPB1432)

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RPPB1432

Description

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Product Name:Mouse ALPL Recombinant Protein
Product Code:RPPB1432
Size:5µg
Species:Mouse
Target:ALPL
Synonyms:Alpl,Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:ALPL protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVTAARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTRCNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQLMHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLALDPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQALHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNGPGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYASCIGANLDHCA WAGSGLEHHH HHH
Biological Activity:Specific activity is > 46,000 pmol/min/ug and isdefined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphateto phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.

AlkalinePhosphatase Liver/Bone/Kidney (Alpl) is a part of the alkaline phosphatasesfamily which comprises 4 related alkaline phosphatases. Alpl is amembrane-bound glycosylated enzyme which is not expressed in any particulartissue. Alpl takes part in skeletal mineralization.

ALPL produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) andhaving a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear atapproximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:This isozyme may play a role in skeletal mineralization.
UniProt Protein Details:
NCBI Summary:This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
UniProt Code:P09242
NCBI GenInfo Identifier:563317855
NCBI Gene ID:11647
NCBI Accession:NP_001274101.1
UniProt Secondary Accession:P09242,Q6P1B0
UniProt Related Accession:P09242
Molecular Weight:57,514 Da
NCBI Full Name:alkaline phosphatase, tissue-nonspecific isozyme preproprotein
NCBI Synonym Full Names:alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol:Alpl��
NCBI Official Synonym Symbols:ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP��
NCBI Protein Information:alkaline phosphatase, tissue-nonspecific isozyme
UniProt Protein Name:Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Synonym Protein Names:Alkaline phosphatase 2; Alkaline phosphatase liver/bone/kidney isozyme
Protein Family:Alkaline proteinase
UniProt Gene Name:Alpl��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P09242
Research Area:
Enzymes
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