Description
| Product Name: | Mouse ALPL Recombinant Protein |
| Product Code: | RPPB1432 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | ALPL |
| Synonyms: | Alpl,Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ALPL protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greaterthan 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVTAARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTRCNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQLMHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLALDPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQALHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNGPGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYASCIGANLDHCA WAGSGLEHHH HHH |
| Biological Activity: | Specific activity is > 46,000 pmol/min/ug and isdefined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphateto phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C. |
AlkalinePhosphatase Liver/Bone/Kidney (Alpl) is a part of the alkaline phosphatasesfamily which comprises 4 related alkaline phosphatases. Alpl is amembrane-bound glycosylated enzyme which is not expressed in any particulartissue. Alpl takes part in skeletal mineralization.
ALPL produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) andhaving a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear atapproximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | This isozyme may play a role in skeletal mineralization. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P09242 |
| NCBI GenInfo Identifier: | 563317855 |
| NCBI Gene ID: | 11647 |
| NCBI Accession: | NP_001274101.1 |
| UniProt Secondary Accession: | P09242,Q6P1B0 |
| UniProt Related Accession: | P09242 |
| Molecular Weight: | 57,514 Da |
| NCBI Full Name: | alkaline phosphatase, tissue-nonspecific isozyme preproprotein |
| NCBI Synonym Full Names: | alkaline phosphatase, liver/bone/kidney |
| NCBI Official Symbol: | Alpl�� |
| NCBI Official Synonym Symbols: | ALP; Akp2; TNAP; Akp-2; APTNAP; TNSALP�� |
| NCBI Protein Information: | alkaline phosphatase, tissue-nonspecific isozyme |
| UniProt Protein Name: | Alkaline phosphatase, tissue-nonspecific isozyme |
| UniProt Synonym Protein Names: | Alkaline phosphatase 2; Alkaline phosphatase liver/bone/kidney isozyme |
| Protein Family: | Alkaline proteinase |
| UniProt Gene Name: | Alpl�� |
| UniProt Entry Name: |
