Enzymes Recombinant Proteins

Mouse ALDH2 Recombinant Protein (RPPB1387)

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RPPB1387

Description

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Product Name:Mouse ALDH2 Recombinant Protein
Product Code:RPPB1387
Size:10µg
Species:Mouse
Target:ALDH2
Synonyms:Aldehyde dehydrogenase, mitochondrial, AHD-M1, ALDH class2, ALDH-E2, ALDHI.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:ALDH2 Mouse protein (0.5mg/ml) is formulated in Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEV ICQVAEGNKE DVDKAVKAARAAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVIS YLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHEPVGVCGQIIP WNFPLLMQAW KLGPALATGN VVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGSTEVGHLIQVAA GSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAK SRVVGNPFDSRTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAK EEIFGPVMQI LKFKTIEEVV GRANDSKYGLAAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGG YKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS
Biological Activity:Specific activity is > 180 pmol/min/ug, and was obtained by measuring the increase of NADH in absorbance at 340 nm resulting from the reduction of NAD at pH 8.0 at 25C.

ALDH2 is part of the aldehyde dehydrogenase family of proteins which catalyze the chemical transformation from acetaldehyde to acetic acid. ALDH2 is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH2 has 2 major liver isoforms: cytosolic and mitochondrial, which differ by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Nearly all Caucasians have 2 major isozymes, whereas roughly 50% of Orientals have only the cytosolic isozyme, omitting the mitochondrial isozyme. The extremely higher rate of acute alcohol intoxication with Orientals compared to Caucasians is due to the fact of the absence of mitochondrial isozyme. ALDH2 has a low Km for acetaldehydes, and is localized in mitochondrial matrix.

ALDH2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 523 amino acids (20-519) and having a molecular mass of 56.8kDa.ALDH2 Mouse is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Is capable of converting retinaldehyde to retinoic acid.
UniProt Protein Details:
NCBI Summary:
UniProt Code:P47738
NCBI GenInfo Identifier:6753036
NCBI Gene ID:11669
NCBI Accession:NP_033786.1
UniProt Secondary Accession:P47738
UniProt Related Accession:P47738
Molecular Weight:56,538 Da
NCBI Full Name:aldehyde dehydrogenase, mitochondrial isoform 1
NCBI Synonym Full Names:aldehyde dehydrogenase 2, mitochondrial
NCBI Official Symbol:Aldh2��
NCBI Official Synonym Symbols:Ahd5; ALDHI; Ahd-5; AHD-M1; ALDH-E2��
NCBI Protein Information:aldehyde dehydrogenase, mitochondrial
UniProt Protein Name:Aldehyde dehydrogenase, mitochondrial
UniProt Synonym Protein Names:AHD-M1; ALDH class 2; ALDH-E2; ALDHI
Protein Family:
UniProt Gene Name:Aldh2��
UniProt Entry Name:
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
P47738
Research Area:
Enzymes
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