Enzymes Recombinant Proteins
Mouse AK1 Recombinant Protein (RPPB2430)
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- SKU:
- RPPB2430
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9R0Y5
- Research Area:
- Enzymes
Description
| Product Name: | Mouse AK1 Recombinant Protein |
| Product Code: | RPPB2430 |
| Size: | 5µg |
| Species: | Mouse |
| Target: | AK1 |
| Synonyms: | Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | AK1 protein solution (0.5mg/ml) containing 20mM Tris-Hcl buffer (pH8.0), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDDNEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK |
| Biological Activity: | Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C. |
AK1 is a small ubiquitous enzyme which is essential for maintenance and cell growth. It is involved in the regulation of adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. The AK1 protein is found in the cytosol of skeletal muscle, brain and erythrocytes. Defects in the AK1 gene are the cause of a form of hemolytic anemia.
AK1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210 a.a) and having a molecular mass of 25.5kDa.AK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. May provide a mechanism to buffer the adenylate energy charge for sperm motility. |
| UniProt Code: | Q9R0Y5 |
| NCBI GenInfo Identifier: | 13959400 |
| NCBI Gene ID: | 11636 |
| NCBI Accession: | Q9R0Y5.1 |
| UniProt Related Accession: | Q9R0Y5 |
| Molecular Weight: | Predicted Molecular Mass: 25.2kDaAccurate Molecular Mass: 25kDa |
| NCBI Full Name: | Adenylate kinase isoenzyme 1 |
| NCBI Synonym Full Names: | adenylate kinase 1 |
| NCBI Official Symbol: | Ak1�� |
| NCBI Official Synonym Symbols: | Ak-1; B430205N08Rik�� |
| NCBI Protein Information: | adenylate kinase isoenzyme 1 |
| UniProt Protein Name: | Adenylate kinase isoenzyme 1 |
| UniProt Synonym Protein Names: | ATP-AMP transphosphorylase 1 |
| Protein Family: | Adenylate kinase |
| UniProt Gene Name: | Ak1�� |
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