Cytokines Recombinant Proteins

Mouse Acrp30 Recombinant Protein (RPPB0021)

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RPPB0021

Description

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Product Name:Mouse Acrp30 Recombinant Protein
Product Code:RPPB0021
Size:10µg
Species:Mouse
Target:Acrp30
Synonyms:Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source:HEK293 Cells
Physical Appearance:Filtered white lyophilized (freeze-dried) powder.
Formulation:Mouse Acrp30 filtered (0.4�m) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2.
Solubility:Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized Acrp30 Mouse at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted Acrp30 Mouse can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Acrp30 Mouse purity is greater than 95% as determined by SDS-PAGE.

Adiponectin is a hormone exclusively expressed from adipose tissue. Many studies demonstrate that adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle, attenuate hepatic lipogenesis and luconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells.In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (MMW, also called trimer) forms 8. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.

Trimeric form of Acrp30 Mouse was expressed in HEK293 cells.The cysteine 39 was replaced with alanine (C39A) 9. mAd-C39A can only form trimer, but not hexamer or HMW form.

UniProt Protein Function:adiponectin: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A via the C1q domain (heterotrimeric complex). Synthesized exclusively by adipocytes and secreted into plasma.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Endoplasmic reticulum; Hormone; Secreted

Cellular Component: extracellular space; cell surface; collagen; protein complex; endoplasmic reticulum; extracellular region

Molecular Function:identical protein binding; protein binding; protein homodimerization activity; hormone activity; sialic acid binding; receptor binding

Biological Process: negative regulation of MAP kinase activity; negative regulation of phagocytosis; negative regulation of smooth muscle cell proliferation; negative regulation of hormone secretion; positive regulation of cellular protein metabolic process; membrane hyperpolarization; negative regulation of smooth muscle cell migration; glucose homeostasis; negative regulation of granulocyte differentiation; positive regulation of interleukin-8 production; positive regulation of glucose import; negative regulation of gluconeogenesis; response to glucose stimulus; adiponectin-mediated signaling pathway; negative regulation of protein amino acid autophosphorylation; negative regulation of blood pressure; negative regulation of cell migration; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of heterotypic cell-cell adhesion; positive regulation of signal transduction; glucose metabolic process; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; negative regulation of synaptic transmission; membrane depolarization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of fatty acid metabolic process; fatty acid beta-oxidation; negative regulation of macrophage differentiation; cellular response to insulin stimulus; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of protein kinase activity; negative regulation of inflammatory response; brown fat cell differentiation; fatty acid oxidation; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of myeloid cell apoptosis; positive regulation of blood pressure

UniProt Code:Q60994
NCBI GenInfo Identifier:408359957
NCBI Gene ID:11450
NCBI Accession:Q60994.2
UniProt Secondary Accession:Q60994,Q62400, Q6GTX4, Q9DC68,
UniProt Related Accession:Q60994
Molecular Weight:26,809 Da
NCBI Full Name:Adiponectin
NCBI Synonym Full Names:adiponectin, C1Q and collagen domain containing
NCBI Official Symbol:Adipoq��
NCBI Official Synonym Symbols:APN; Acdc; apM1; 30kDa; GBP28; adipo; Acrp30��
NCBI Protein Information:adiponectin; adipocyte-specific protein AdipoQ; adipocyte complement related protein; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein
UniProt Protein Name:Adiponectin
UniProt Synonym Protein Names:30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ
Protein Family:Adiponectin
UniProt Gene Name:Adipoq��
UniProt Entry Name:ADIPO_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q60994
Research Area:
Cytokines
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