Enzymes Recombinant Proteins

Mouse ACE2 Recombinant Protein (RPPB1329)

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RPPB1329

Description

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Product Name:Mouse ACE2 Recombinant Protein
Product Code:RPPB1329
Size:10µg
Species:Mouse
Target:ACE2
Synonyms:ACE2, 2010305L05Rik, Angiotensin I Converting Enzyme, Angiotensin I Converting, Enzyme (Peptidyl-Dipeptidase A), Angiotensin-Converting Enzyme Homolog, Angiotensin-Converting Enzyme, ACE-Related Carboxypeptidase, Metalloprotease MPROT15, Peptidyl-Dipeptidase A, ACEH, EC 3.4.17.23, EC 3.4.17.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ACE2 solution contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:QSLTEENAKT FLNNFNQEAE DLSYQSSLAS WNYNTNITEE NAQKMSEAAA KWSAFYEEQS�KTAQSFSLQE IQTPIIKRQL QALQQSGSSA LSADKNKQLN TILNTMSTIY STGKVCNPKN�PQECLLLEPG LDEIMATSTD YNSRLWAWEG WRAEVGKQLR PLYEEYVVLK NEMARANNYN�DYGDYWRGDY EAEGADGYNY NRNQLIEDVE RTFAEIKPLY EHLHAYVRRK LMDTYPSYIS�PTGCLPAHLL GDMWGRFWTN LYPLTVPFAQ KPNIDVTDAM MNQGWDAERI FQEAEKFFVS�VGLPHMTQGF WANSMLTEPA DGRKVVCHPT AWDLGHGDFR IKMCTKVTMD�NFLTAHHEMG HIQYDMAYAR QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLPSD�FQEDSETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFRGE IPKEQWMKKW WEMKREIVGV�VEPLPHDETY CDPASLFHVS NDYSFIRYYT RTIYQFQFQE ALCQAAKYNG SLHKCDISNS�TEAGQKLLKM LSLGNSEPWT KALENVVGAR NMDVKPLLNY FQPLFDWLKE QNRNSFVGWN�TEWSPYADQS IKVRISLKSA LGANAYEWTN NEMFLFRSSV AYAMRKYFSI IKNQTVPFLE�EDVRVSDLKP RVSFYFFVTS PQNVSDVIPR SEVEDAIRMS RGRINDVFGL NDNSLEFLGI�HPTLEPPYQPPVTLEHHHHH H

ACE-2 (Angiotensin converting enzyme 2) an enzyme bound to cell membranes in various organs such as intestines arteries , lungs, heart & kidney. ACE2 an entry receptor of SARS coronaviruses as well as SARS-CoV-2,.The coronavirus spike (S) glycoprotein is a class I viral fusion antigen located on the external envelope of the virion that takes part in a critical part in viral infection by identifying host cell receptors and facilitating fusion of the viral and cellular membranes. 2 main domains in coronavirus S1 have been recognized, the N-terminal domain and C-terminal domain. One or the other and/or both S1 domains function as a receptor-binding domain.� SARS-CoV + MERS-CoV equally use C-domain to attach their receptors.ACE2 is a type I transmembrane antigen with an extracellular N-terminal domain having the catalytic site and an intracellular C-terminal tail. ACE2 obtains a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing an HEXXH zinc-binding domain. ACE-2 plays a role as a mono-carboxypeptidase which degrades Ang I to produce the nonapeptide Ang 1�9 and Ang II to create the heptapeptide Ang 1�7.

ACE2 Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 731 amino acids (18-740 aa) and having a molecular mass of 84.5kDa. ACE2 is fused to an 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:ACE2: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Interacts with ITGB1. Interacts with SARS-CoV and HCoV- NL63 spike glycoprotein. Up-regulated in failing heart. Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells. Expressed in heart, kidney, testis, and gastrointestinal system. Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. Belongs to the peptidase M2 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.4.17.23; Protease; Membrane protein, integralCellular Component: extracellular space; cell surface; membrane; cytoplasm; integral to membrane; extracellular region; plasma membraneMolecular Function: viral receptor activity; peptidase activity; peptidyl-dipeptidase activity; hydrolase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding; endopeptidase activity; glycoprotein bindingBiological Process: virion attachment, binding of host cell surface receptor; negative regulation of smooth muscle cell proliferation; receptor biosynthetic process; proteolysis; angiotensin mediated drinking behavior; regulation of systemic arterial blood pressure by renin-angiotensin
UniProt Protein Details:
NCBI Summary:
UniProt Code:Q8R0I0
NCBI GenInfo Identifier:71152217
NCBI Gene ID:70008
NCBI Accession:Q8R0I0.1
UniProt Secondary Accession:Q8R0I0,Q99N70, Q99N71
UniProt Related Accession:Q8R0I0
Molecular Weight:40,340 Da
NCBI Full Name:Angiotensin-converting enzyme 2
NCBI Synonym Full Names:angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
NCBI Official Symbol:Ace2��
NCBI Official Synonym Symbols:2010305L05Rik��
NCBI Protein Information:angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme 2
UniProt Protein Name:Angiotensin-converting enzyme 2
UniProt Synonym Protein Names:ACE-related carboxypeptidaseCleaved into the following chain:Processed angiotensin-converting enzyme 2
Protein Family:Angiotensin-converting enzyme
UniProt Gene Name:Ace2��
UniProt Entry Name:ACE2_MOUSE
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Additional Information

Product Type:
Recombinant Protein
Species:
Mouse
Uniprot:
Q8R0I0
Research Area:
Enzymes
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