Description
Product Name: | MonoMethyl-Histone H3-K36 Monoclonal Antibody |
Product Code: | CAB23944 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Applications: | Western blotting, Immunoprecipitation |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Clone No: | ARC61490 |
Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
Tested Applications: | WB, IP, ChIP, ELISA, DB |
Key Applications: | Western blotting, Immunoprecipitation |
Recommended Dilution: | WB 1:1000-1:5000, IP 1:50-1:200, ChIP 1:50-1:200, DB 1:1000-1:5000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Positive Samples: | HeLa, NIH/3T3 |
Cellular Location: | Chromosome, Nucleus |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Immunogen: | A synthetic monomethylated peptide around K36 of human histone H3 (NP_003520.1). |
Sequence: | MARTK QTARK STGGK APRKQ LATKA ARKSA PATGG VKKPH RYRPG TVALR EIRRY QKSTE LLIRK LPFQR LVREI AQDFK TDLRF QSSAV MALQE ACEAY |
Synonyms: | H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
Calculated MW: | 15kDa |
Observed MW: | 17kDa |