Description
Product Name: | Monomeric SA Recombinant Protein (His tag) |
Product Code: | RPES6800 |
Size: | 20µg |
Species: | Rhizavidin |
Expression Host: | E.coli |
Synonyms: | mSA, Monomeric SA |
Mol Mass: | 12.5 kDa |
AP Mol Mass: | 13-15 kDa |
Tag: | N-His |
Purity: | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin Level: | Please contact us for more information. |
Bio Activity: | Testing in progress |
Sequence: | AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPYTLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATEQGQDTFTKVK |
Storage: | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation: | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the print |
Reconstitution: | Please refer to the printed manual for detailed information. |
Background: | Forms a non-covalent specific complex with biotin. Monomeric streptavidin has unique structural and functional properties that can lead to development of new applications based on the well-known protein–ligand interaction. For example, it allows detection of biotinylated ligands while avoiding target aggregation caused by multivalent binding. Since clustering of biologically active molecules often results in functional changes, monomeric streptavidin can facilitate imaging and tracking studies in live cells by ensuring monovalent biotin binding. |