MOGAT1 Antibody (PACO59277)
- SKU:
- PACO59277
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MOGAT1 Antibody (PACO59277) |
| Antibody SKU: | PACO59277 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human 2-acylglycerol O-acyltransferase 1 protein (163-246AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59277 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO59277 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59277 diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine (By similarity). |
| Synonyms: | 2-acylglycerol O-acyltransferase 1 (EC 2.3.1.22) (Acyl-CoA:monoacylglycerol acyltransferase 1) (MGAT1) (Diacylglycerol O-acyltransferase candidate 2) (hDC2) (Diacylglycerol acyltransferase 2-like protein 1) (Monoacylglycerol O-acyltransferase 1), MOGAT1, DC2 DGAT2L1 |
| UniProt Protein Function: | MOGAT1: Catalyzes the formation of diacylglycerol from 2- monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine. Belongs to the diacylglycerol acyltransferase family.Protein type: Endoplasmic reticulum; Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.22Chromosomal Location of Human Ortholog: 2q36.1Cellular Component: endoplasmic reticulum membraneMolecular Function: 2-acylglycerol O-acyltransferase activityBiological Process: triacylglycerol biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerols, the precursor of physiologically important lipids such as triacylglycerol and phospholipids (Yen et al., 2002 [PubMed 12077311]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q96PD6 |
| NCBI GenInfo Identifier: | 292495019 |
| NCBI Gene ID: | 116255 |
| NCBI Accession: | Q96PD6.2 |
| UniProt Secondary Accession: | Q96PD6,Q6IEE5 |
| UniProt Related Accession: | Q96PD6 |
| Molecular Weight: | 38,812 Da |
| NCBI Full Name: | 2-acylglycerol O-acyltransferase 1 |
| NCBI Synonym Full Names: | monoacylglycerol O-acyltransferase 1 |
| NCBI Official Symbol: | MOGAT1 |
| NCBI Official Synonym Symbols: | MGAT1; DGAT2L; DGAT2L1 |
| NCBI Protein Information: | 2-acylglycerol O-acyltransferase 1 |
| UniProt Protein Name: | 2-acylglycerol O-acyltransferase 1 |
| UniProt Synonym Protein Names: | Acyl-CoA:monoacylglycerol acyltransferase 1; MGAT1; Diacylglycerol O-acyltransferase candidate 2; hDC2; Diacylglycerol acyltransferase 2-like protein 1; Monoacylglycerol O-acyltransferase 1 |
| Protein Family: | 2-acylglycerol O-acyltransferase |
| UniProt Gene Name: | MOGAT1 |
| UniProt Entry Name: | MOGT1_HUMAN |
| Antibodies |
| MOGAT1 Antibody (PACO10543) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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