Anti-MOCS2 Antibody (PACO60945)

Name: MOCS2 Antibody (PACO60945)
SKU: PACO60945
Availability: OutOfStock

Antibody Name:	MOCS2 Antibody (PACO60945)
Antibody SKU:	PACO60945
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Molybdopterin synthase sulfur carrier subunit protein (1-88AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Immunofluorescence staining of HepG2 cells with PACO60945 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.

Synonyms:

Molybdopterin synthase sulfur carrier subunit (MOCO1-A) (Molybdenum cofactor synthesis protein 2 small subunit) (Molybdenum cofactor synthesis protein 2A) (MOCS2A) (Molybdopterin-synthase small subunit) (Sulfur carrier protein MOCS2A), MOCS2, MOCO1

UniProt Protein Function:	Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group.
NCBI Summary:	Eukaryotic molybdoenzymes use a unique molybdenum cofactor (MoCo) consisting of a pterin, termed molybdopterin, and the catalytically active metal molybdenum. MoCo is synthesized from precursor Z by the heterodimeric enzyme molybdopterin synthase. The large and small subunits of molybdopterin synthase are both encoded from this gene by overlapping open reading frames. The proteins were initially thought to be encoded from a bicistronic transcript. They are now thought to be encoded from monocistronic transcripts. Alternatively spliced transcripts have been found for this locus that encode the large and small subunits. [provided by RefSeq, Jul 2008]
UniProt Code:	O96033
NCBI GenInfo Identifier:	4758732
NCBI Gene ID:	4338
NCBI Accession:	NP_004522
UniProt Secondary Accession:	O96033,Q6IAI3,
UniProt Related Accession:	O96007,O96033
Molecular Weight:	25 kDa (224aa) confirmed by MALDI-TOF
NCBI Full Name:	molybdopterin synthase catalytic subunit large subunit MOCS2B
NCBI Synonym Full Names:	molybdenum cofactor synthesis 2
NCBI Official Symbol:	MOCS2
NCBI Official Synonym Symbols:	MPTS; MCBPE; MOCO1; MOCODB
NCBI Protein Information:	molybdopterin synthase catalytic subunit; molybdopterin synthase sulfur carrier subunit
UniProt Protein Name:	Molybdopterin synthase catalytic subunit
UniProt Synonym Protein Names:	MOCO1-B; Molybdenum cofactor synthesis protein 2 large subunit
UniProt Gene Name:	MOCS2