MOB1B Antibody (PACO37126)
- SKU:
- PACO37126
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MOB1B Antibody (PACO37126) |
| Antibody SKU: | PACO37126 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:2000, IF:1:200-1:500, IP:1:200-1:2000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human MOB kinase activator 1B protein (2-216AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of NIH/3T3 cells with PACO37126 at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO37126 diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunoprecipitating MOB1B in K562 whole cell lysate. Lane 1: Rabbit control IgG (1µg) instead of PACO37126 in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO37126 (6µg) + K562 whole cell lysate (500µg). Lane 3: K562 whole cell lysate (10µg). |
| Background: | Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L. |
| Synonyms: | MOB kinase activator 1B (Mob1 homolog 1A) (Mob1A) (Mob1B) (Mps one binder kinase activator-like 1A), MOB1B, MOB4A MOBKL1A |
| UniProt Protein Function: | MOB1B: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L. Binds STK38L. Interacts with LATS1 and LATS2. Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver. Belongs to the MOB1/phocein family. |
| UniProt Protein Details: | Protein type:Protein kinase, regulatory subunit; Activator Chromosomal Location of Human Ortholog: 4q13.3 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:kinase activator activity; kinase binding; protein binding Biological Process: regulation of protein amino acid autophosphorylation |
| NCBI Summary: | The protein encoded by this gene is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q7L9L4 |
| NCBI GenInfo Identifier: | 56749324 |
| NCBI Gene ID: | 92597 |
| NCBI Accession: | Q7L9L4.3 |
| UniProt Secondary Accession: | Q7L9L4,Q8IY23, B2R8U6, B4DRY3, |
| UniProt Related Accession: | Q7L9L4 |
| Molecular Weight: | 25,499 Da |
| NCBI Full Name: | MOB kinase activator 1B |
| NCBI Synonym Full Names: | MOB kinase activator 1B |
| NCBI Official Symbol: | MOB1B |
| NCBI Official Synonym Symbols: | MATS2; MOB4A; MOBKL1A |
| NCBI Protein Information: | MOB kinase activator 1B |
| UniProt Protein Name: | MOB kinase activator 1B |
| UniProt Synonym Protein Names: | Mob1 homolog 1A; Mob1A; Mob1B; Mps one binder kinase activator-like 1A |
| Protein Family: | MOB kinase activator |
| UniProt Gene Name: | MOB1B |
| UniProt Entry Name: | MOB1B_HUMAN |
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