Name: MMP28 Antibody (PACO56194)
SKU: PACO56194
Availability: OutOfStock

Antibody Name:	MMP28 Antibody (PACO56194)
Antibody SKU:	PACO56194
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Matrix metalloproteinase-28 protein (351-475AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: MMP28 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 15 kDa. Observed band size: 59 kDa.
	IHC image of PACO56194 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56194 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Can degrade casein. Could play a role in tissues homeostasis and repair.
Synonyms:	Matrix metalloproteinase-28 (MMP-28) (EC 3.4.24) (Epilysin), MMP28, MMP25

UniProt Protein Function:	MMP28: Can degrade casein. Could play a role in tissues homeostasis and repair. Belongs to the peptidase M10A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Protease; EC 3.4.24.-; Secreted; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q12 Cellular Component: proteinaceous extracellular matrix; cytoplasm Molecular Function:zinc ion binding; metalloendopeptidase activity; calcium ion binding Biological Process: proteolysis
NCBI Summary:	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction and tissue remodeling, and disease processes, such as asthma and metastasis. This gene encodes a secreted enzyme that degrades casein. Its expression pattern suggests that it plays a role in tissue homeostasis and in wound repair. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:	Q9H239
NCBI GenInfo Identifier:	37538314
NCBI Gene ID:	79148
NCBI Accession:	Q9H239.2
UniProt Secondary Accession:	Q9H239,Q96F04, Q96TE2,
UniProt Related Accession:	Q9H239
Molecular Weight:	520
NCBI Full Name:	Matrix metalloproteinase-28
NCBI Synonym Full Names:	matrix metallopeptidase 28
NCBI Official Symbol:	MMP28
NCBI Official Synonym Symbols:	MM28; MMP25; MMP-25; MMP-28; EPILYSIN
NCBI Protein Information:	matrix metalloproteinase-28; matrix metalloprotease MMP25
UniProt Protein Name:	Matrix metalloproteinase-28
UniProt Synonym Protein Names:	Epilysin
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP28
UniProt Entry Name:	MMP28_HUMAN

Antibodies	ELISA Kits
MMP28 Antibody (PACO10526)	Human MMP28 / Matrix metalloproteinase-28 ELISA Kit
	Human MMP25 ELISA Kit
	Human MMP-28 (Matrix Metalloproteinase 28) CLIA Kit (HUES00871)
	Human Matrix metalloproteinase-28 (MMP28) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)