Antibody Name:	Mmp2 Antibody (PACO64303)
Antibody SKU:	PACO64303
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	WB:1:1000-1:5000
Species Reactivity:	Mouse, Human
Immunogen:	Recombinant Mouse 72 kDa type IV collagenase protein (412-662AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: Mmp2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 75, 66 kDa. Observed band size: 75 kDa.

Background:

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- |-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3β in vitro. Involved in the formation of the fibrovascular tissues (By similarity).By similarityPEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).By similarityIsoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.

Synonyms:

72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) [Cleaved into: PEX], Mmp2

UniProt Protein Function:	MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-\|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:Protease; Secreted; Apoptosis; Cell development/differentiation; EC 3.4.24.24; Secreted, signal peptide; Motility/polarity/chemotaxis Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; sarcomere; membrane; mitochondrion; cytoplasm; plasma membrane; extracellular region; nucleus Molecular Function:peptidase activity; zinc ion binding; hydrolase activity; metallopeptidase activity; metal ion binding; metalloendopeptidase activity; fibronectin binding Biological Process: response to drug; cell migration; intramembranous ossification; positive regulation of apoptosis; tissue remodeling; negative regulation of cell adhesion; proteolysis; axon regeneration in the peripheral nervous system; blood vessel maturation; negative regulation of vasoconstriction; collagen catabolic process; response to hydrogen peroxide; ovarian follicle development; response to estrogen stimulus; skeletal morphogenesis; response to mechanical stimulus; positive regulation of innate immune response; response to hypoxia; response to oxidative stress; angiogenesis; embryo implantation; positive regulation of cell migration
UniProt Code:	P33434
NCBI GenInfo Identifier:	461766
NCBI Gene ID:	17390
NCBI Accession:	P33434.1
UniProt Related Accession:	P33434
Molecular Weight:	65,883 Da
NCBI Full Name:	72 kDa type IV collagenase
NCBI Synonym Full Names:	matrix metallopeptidase 2
NCBI Official Symbol:	Mmp2
NCBI Official Synonym Symbols:	GelA; Clg4a; MMP-2
NCBI Protein Information:	72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2
UniProt Protein Name:	72 kDa type IV collagenase
UniProt Synonym Protein Names:	72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	Mmp2
UniProt Entry Name:	MMP2_MOUSE