MKX Antibody (PACO58284)
- SKU:
- PACO58284
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MKX Antibody (PACO58284) |
| Antibody SKU: | PACO58284 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Homeobox protein Mohawk protein (157-305AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate. All lanes: MKX antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa. |
 | Immunofluorescence staining of Hela cells with PACO58284 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO58284 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | May act as a morphogenetic regulator of cell adhesion. |
| Synonyms: | Homeobox protein Mohawk, MKX, C10orf48 IRXL1 |
| UniProt Protein Function: | MKX: May act as a morphogenetic regulator of cell adhesion. Belongs to the TALE/IRO homeobox family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 10p12.1Biological Process: muscle development |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is an IRX family-related homeobox protein that may play a role in cell adhesion. Studies in mice suggest that this protein may be a regulator of tendon development. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jun 2011] |
| UniProt Code: | Q8IYA7 |
| NCBI GenInfo Identifier: | 296437371 |
| NCBI Gene ID: | 283078 |
| NCBI Accession: | Q8IYA7.2 |
| UniProt Secondary Accession: | Q8IYA7,B3KWM5 |
| UniProt Related Accession: | Q8IYA7 |
| Molecular Weight: | 39,331 Da |
| NCBI Full Name: | Homeobox protein Mohawk |
| NCBI Synonym Full Names: | mohawk homeobox |
| NCBI Official Symbol: | MKX |
| NCBI Official Synonym Symbols: | IFRX; IRXL1; C10orf48 |
| NCBI Protein Information: | homeobox protein Mohawk |
| UniProt Protein Name: | Homeobox protein Mohawk |
| UniProt Synonym Protein Names: | |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | MKX |
| UniProt Entry Name: | MKX_HUMAN |
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