MKRN1 Antibody (PACO56202)
- SKU:
- PACO56202
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MKRN1 Antibody (PACO56202) |
| Antibody SKU: | PACO56202 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human E3 ubiquitin-protein ligase makorin-1 protein (109-209AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56202 diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. |
| Synonyms: | E3 ubiquitin-protein ligase makorin-1 (EC 2.3.2.27) (RING finger protein 61) (RING-type E3 ubiquitin transferase makorin-1), MKRN1, RNF61 |
| UniProt Protein Function: | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein that belongs to a novel class of zinc finger proteins. The encoded protein functions as a transcriptional co-regulator, and as an E3 ubiquitin ligase that promotes the ubiquitination and proteasomal degradation of target proteins. The protein encoded by this gene is thought to regulate RNA polymerase II-catalyzed transcription. Substrates for this protein's E3 ubiquitin ligase activity include the capsid protein of the West Nile virus and the catalytic subunit of the telomerase ribonucleoprotein. This protein controls cell cycle arrest and apoptosis by regulating p21, a cell cycle regulator, and the tumor suppressor protein p53. Pseudogenes of this gene are present on chromosomes 1, 3, 9, 12 and 20, and on the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014] |
| UniProt Code: | Q9UHC7 |
| NCBI GenInfo Identifier: | 223468622 |
| NCBI Gene ID: | 23608 |
| NCBI Accession: | NP_001138597.1 |
| UniProt Secondary Accession: | Q9UHC7,Q256Y7, Q59G11, Q6GSF1, Q9H0G0, Q9UEZ7, Q9UHW2 A4D1T7, B3KXB4 |
| UniProt Related Accession: | Q9UHC7 |
| Molecular Weight: | 35,224 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase makorin-1 isoform 2 |
| NCBI Synonym Full Names: | makorin ring finger protein 1 |
| NCBI Official Symbol: | MKRN1 |
| NCBI Official Synonym Symbols: | RNF61 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase makorin-1 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase makorin-1 |
| UniProt Synonym Protein Names: | RING finger protein 61; RING-type E3 ubiquitin transferase makorin-1Curated |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | MKRN1 |
| UniProt Entry Name: |
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