MID1IP1 Antibody (PACO56182)
- SKU:
- PACO56182
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MID1IP1 Antibody (PACO56182) |
| Antibody SKU: | PACO56182 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Mid1-interacting protein 1 protein (1-183AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56182 diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO56182 at 1:125, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO56182 diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules (By similarity). |
| Synonyms: | Mid1-interacting protein 1 (Gastrulation-specific G12-like protein) (Mid1-interacting G12-like protein) (Protein STRAIT11499) (Spot 14-related protein) (S14R) (Spot 14-R), MID1IP1, MIG12 |
| UniProt Protein Function: | MID1IP1: Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules. Belongs to the SPOT14 family.Chromosomal Location of Human Ortholog: Xp11.4Cellular Component: cytosol; microtubule cytoskeletonMolecular Function: protein bindingBiological Process: negative regulation of microtubule depolymerization; positive regulation of fatty acid biosynthetic process; positive regulation of ligase activity; protein polymerization; regulation of lipid biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9NPA3 |
| NCBI GenInfo Identifier: | 21759081 |
| NCBI Gene ID: | 58526 |
| NCBI Accession: | Q9NPA3.1 |
| UniProt Secondary Accession: | Q9NPA3,D3DWB2 |
| UniProt Related Accession: | Q9NPA3 |
| Molecular Weight: | 20,202 Da |
| NCBI Full Name: | Mid1-interacting protein 1 |
| NCBI Synonym Full Names: | MID1 interacting protein 1 |
| NCBI Official Symbol: | MID1IP1 |
| NCBI Official Synonym Symbols: | S14R; MIG12; THRSPL; G12-like; STRAIT11499 |
| NCBI Protein Information: | mid1-interacting protein 1 |
| UniProt Protein Name: | Mid1-interacting protein 1 |
| UniProt Synonym Protein Names: | Gastrulation-specific G12-like protein; Mid1-interacting G12-like protein; Protein STRAIT11499; Spot 14-related protein; S14R; Spot 14-R |
| Protein Family: | Mid1-interacting protein |
| UniProt Gene Name: | MID1IP1 |
| UniProt Entry Name: | M1IP1_HUMAN |
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