Name: MFNG Antibody (PACO62943)
SKU: PACO62943
Availability: OutOfStock

Antibody Name:	MFNG Antibody (PACO62943)
Antibody SKU:	PACO62943
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Beta-1,3-N-acetylglucosaminyltransferase manic fringe protein (28-321AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO62943 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity.
Synonyms:	Beta-1,3-N-acetylglucosaminyltransferase manic fringe (EC 2.4.1.222) (O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase), MFNG

UniProt Protein Function:	MFNG: Glycosyltransferase involved in the elongation of O- linked ligands to activate Notch signaling. Possesses fucose- specific beta-1,3-N-acetylglucosaminyltransferase activity. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; EC 2.4.1.222Chromosomal Location of Human Ortholog: 22q12Cellular Component: extracellular space; integral to Golgi membraneMolecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activityBiological Process: positive regulation of protein binding; metabolic process; pattern specification process; positive regulation of Notch signaling pathway
UniProt Protein Details:
NCBI Summary:	This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]
UniProt Code:	O00587
NCBI GenInfo Identifier:	21536461
NCBI Gene ID:	4242
NCBI Accession:	NP_002396
UniProt Secondary Accession:	O00587
UniProt Related Accession:	O00587
Molecular Weight:	36kDa
NCBI Full Name:	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform 1
NCBI Synonym Full Names:	MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol:	MFNG
NCBI Official Synonym Symbols:
NCBI Protein Information:	beta-1,3-N-acetylglucosaminyltransferase manic fringe
UniProt Protein Name:	Beta-1,3-N-acetylglucosaminyltransferase manic fringe
UniProt Synonym Protein Names:	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Protein Family:	Beta-1,3-N-acetylglucosaminyltransferase manic fringe
UniProt Gene Name:	MFNG
UniProt Entry Name:	MFNG_HUMAN