Cardiovascular Recombinant Proteins
MFG-E8/lactadherin/MFGE8 Recombinant Protein (RPES2684)
- SKU:
- RPES2684
Description
MFG-E8/lactadherin/MFGE8 Recombinant Protein
MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.
| Product Name: | MFG-E8/lactadherin/MFGE8 Recombinant Protein (RPES2684) |
| Product Code: | RPES2684 |
| Size: | 50µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | BA46,EDIL1,HMFG,hP47,HsT19888,MFG-E8,MFGM,OAcGD3S,SED1,SPAG10 |
| Accession: | Q08431-1 |
| Sequence: | Met 1-Cys 387 |
| Fusion tag: | C-His |
| Activity: | When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 µg/mL, 100 ?L/well), 45-85% cells will adhere after 1 hour at 37?. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human MFGE8 isoform 1 (Q08431-1) (Met 1-Cys 387) was fused with a polyhistidine tag at the C-terminus. |
| Purity: | > 80 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 42 kDa |
| AP Mol Mass: | 45 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Cardiovascular |
Related Products
Human MFG-E8 / Lactadherin ELISA Kit
Mouse Mfge8(Lactadherin) ELISA Kit
