MEGF9 Antibody (PACO57488)
- SKU:
- PACO57488
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MEGF9 Antibody (PACO57488) |
| Antibody SKU: | PACO57488 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Multiple epidermal growth factor-like domains protein 9 protein (49-178AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate. All lanes: MEGF9 antibody at 11.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 63 kDa. Observed band size: 63 kDa. |
 | Immunofluorescence staining of A549 cells with PACO57488 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO57488 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Synonyms: | Multiple epidermal growth factor-like domains protein 9 (Multiple EGF-like domains protein 9) (Epidermal growth factor-like protein 5) (EGF-like protein 5), MEGF9, EGFL5 KIAA0818 |
| UniProt Protein Function: | MEGF9: Protein type: Membrane protein, integralChromosomal Location of Human Ortholog: 9q32-q33.3 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9H1U4 |
| NCBI GenInfo Identifier: | 116242627 |
| NCBI Gene ID: | 1955 |
| NCBI Accession: | Q9H1U4.3 |
| UniProt Secondary Accession: | Q9H1U4,O75098, B7Z315 |
| UniProt Related Accession: | Q9H1U4 |
| Molecular Weight: | 62,984 Da |
| NCBI Full Name: | Multiple epidermal growth factor-like domains protein 9 |
| NCBI Synonym Full Names: | multiple EGF like domains 9 |
| NCBI Official Symbol: | MEGF9 |
| NCBI Official Synonym Symbols: | EGFL5 |
| NCBI Protein Information: | multiple epidermal growth factor-like domains protein 9 |
| UniProt Protein Name: | Multiple epidermal growth factor-like domains protein 9 |
| UniProt Synonym Protein Names: | Epidermal growth factor-like protein 5; EGF-like protein 5 |
| Protein Family: | Multiple epidermal growth factor-like domains protein |
| UniProt Gene Name: | MEGF9 |
| UniProt Entry Name: | MEGF9_HUMAN |
| Antibodies |
| MEGF9 Antibody (PACO00741) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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