MED8 Antibody (PACO22405)
- SKU:
- PACO22405
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MED8 Antibody (PACO22405) |
| Antibody SKU: | PACO22405 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from Internal of human MED8. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from Jurkat/K562 cells, using MED8 antibody. |
| Background: | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. |
| Synonyms: | Activator-recruited cofactor 32 kDa component; ARC32; MED8; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8 |
| UniProt Protein Function: | MED8: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. Belongs to the Mediator complex subunit 8 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 1p34.2Cellular Component: nucleoplasm; Srb-mediator complexMolecular Function: protein bindingBiological Process: transcription initiation from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013] |
| UniProt Code: | Q96G25 |
| NCBI GenInfo Identifier: | 31076772 |
| NCBI Gene ID: | 112950 |
| NCBI Accession: | Q96G25.2 |
| UniProt Secondary Accession: | Q96G25,Q5JUY8, Q96FQ4, A9IZ91, A9IZ92 |
| UniProt Related Accession: | Q96G25 |
| Molecular Weight: | 19,002 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 8 |
| NCBI Synonym Full Names: | mediator complex subunit 8 |
| NCBI Official Symbol: | MED8 |
| NCBI Official Synonym Symbols: | ARC32 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 8 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 8 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED8 |
| UniProt Entry Name: | MED8_HUMAN |
| Antibodies |
| MED8 Antibody (PACO02420) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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