Description
Product Name: | Med25 Polyclonal Antibody |
Product Code: | CAB21274 |
Reactivity: | Arabidopsis thaliana |
Applications: | Western blotting |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Reactivity: | Arabidopsis thaliana |
Tested Applications: | WB, ELISA |
Key Applications: | Western blotting |
Recommended Dilution: | WB 1:500-1:1000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
Positive Samples: | Seedling, Inflorescences, Before inflorescence, Rosette leaf |
Cellular Location: | nucleus |
Encodes a nuclear protein that acts in a phyB pathway (but downstream of phyB) and induces flowering in response to suboptimal light conditions. PFT1 promotes flowering in CO dependent and independent pathways and integrates several environmental stimuli, such as light quality and JA-dependent defenses. Mutants are hypo-responsive to far-red and hyper-responsive to red light and flower late under long day conditions. Also shown to be a Mediator subunit regulating jasmonate-dependent defense.
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of arabidopsis thaliana Med25 (NP_173925.3). |
Sequence: | AGKPN QQSAD LSIDT AKNTF YLVLI SENFV EACAA LSHSA TNLPQ TQSPV KVDRA TVAPS IPVTG QPPAP VSSAN GPIQN RQPVS VGPVP TATVK VEPST V |
Synonyms: | F2J7.4, F2J7_4, MED25, mediator 25, PHYTOCHROME AND FLOWERING TIME 1 |
Calculated MW: | 90kDa |
Observed MW: | 75kDa |