Antibody Name:	MED16 Antibody (PACO17237)
Antibody SKU:	PACO17237
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100
Species Reactivity:	Human, Mouse
Immunogen:	Fusion protein of human MED16

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17237(MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).
	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hepg2 and hela cell, Primary antibody: PACO17237(MED16 Antibody) at dilution 1/400 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17237(MED16 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200).

Background:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Synonyms:

mediator complex subunit 16

UniProt Protein Function:	MED16: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 16 family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Receptor, misc.Chromosomal Location of Human Ortholog: 19p13.3Cellular Component: membrane; nucleoplasm; Srb-mediator complexMolecular Function: protein binding; receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activityBiological Process: androgen receptor signaling pathway; positive regulation of transcription, DNA-dependent; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9Y2X0
NCBI GenInfo Identifier:	62511180
NCBI Gene ID:	10025
NCBI Accession:	Q9Y2X0.2
UniProt Secondary Accession:	Q9Y2X0,Q6PJT2, Q96AD4, Q96I35, Q9Y652
UniProt Related Accession:	Q9Y2X0
Molecular Weight:	72,967 Da
NCBI Full Name:	Mediator of RNA polymerase II transcription subunit 16
NCBI Synonym Full Names:	mediator complex subunit 16
NCBI Official Symbol:	MED16
NCBI Official Synonym Symbols:	DRIP92; THRAP5; TRAP95
NCBI Protein Information:	mediator of RNA polymerase II transcription subunit 16
UniProt Protein Name:	Mediator of RNA polymerase II transcription subunit 16
UniProt Synonym Protein Names:	Mediator complex subunit 16; Thyroid hormone receptor-associated protein 5; Thyroid hormone receptor-associated protein complex 95 kDa component; Trap95; Vitamin D3 receptor-interacting protein complex 92 kDa component; DRIP92
Protein Family:	Putative mediator of RNA polymerase II transcription
UniProt Gene Name:	MED16
UniProt Entry Name:	MED16_HUMAN