Name: MBNL3 Antibody (PACO59389)
SKU: PACO59389
Availability: OutOfStock

Antibody Name:	MBNL3 Antibody (PACO59389)
Antibody SKU:	PACO59389
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Muscleblind-like protein 3 protein (116-211AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO59389 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with PACO59389 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.

Synonyms:

Muscleblind-like protein 3 (Cys3His CCG1-required protein) (Muscleblind-like X-linked protein) (Protein HCHCR), MBNL3, CHCR MBLX39 MBXL

UniProt Protein Function:	MBNL3: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction. Belongs to the muscleblind family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: Xq26.2Molecular Function: protein bindingBiological Process: regulation of RNA splicing
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the muscleblind-like family of proteins. The encoded protein may function in regulation of alternative splicing and may play a role in the pathophysiology of myotonic dystrophy. Alternatively spliced transcript variants have been described. [provided by RefSeq, Dec 2009]
UniProt Code:	Q9NUK0
NCBI GenInfo Identifier:	26396510
NCBI Gene ID:	55796
NCBI Accession:	Q9NUK0.2
UniProt Secondary Accession:	Q9NUK0,Q5JXN8, Q5JXN9, Q5JXP4, Q6UDQ1, Q8IUR4, Q8TAD9 Q8TAF4, Q9H0Z7, Q9UF37
UniProt Related Accession:	Q9NUK0
Molecular Weight:	27,687 Da
NCBI Full Name:	Muscleblind-like protein 3
NCBI Synonym Full Names:	muscleblind like splicing regulator 3
NCBI Official Symbol:	MBNL3
NCBI Official Synonym Symbols:	CHCR; MBLX; MBXL; MBLX39
NCBI Protein Information:	muscleblind-like protein 3
UniProt Protein Name:	Muscleblind-like protein 3
UniProt Synonym Protein Names:	Cys3His CCG1-required protein; Muscleblind-like X-linked protein; Protein HCHCR
Protein Family:	Muscleblind-like protein
UniProt Gene Name:	MBNL3
UniProt Entry Name:	MBNL3_HUMAN