MAP3K6 Antibody (PACO55614)
- SKU:
- PACO55614
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MAP3K6 Antibody (PACO55614) |
| Antibody SKU: | PACO55614 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Mitogen-activated protein kinase kinase kinase 6 protein (905-990AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO55614 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of MCF-7 cells with PACO55614 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Component of a protein kinase signal transduction cascade. Activates the JNK, but not ERK or p38 kinase pathways. |
| Synonyms: | Mitogen-activated protein kinase kinase kinase 6 (EC 2.7.11.25) (Apoptosis signal-regulating kinase 2), MAP3K6, ASK2 MAPKKK6 MEKK6 |
| UniProt Protein Function: | MEKK6: a member of the STE11 protein kinase family. Activates JNK, but not ERK or p38. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; STE group; STE11 familyChromosomal Location of Human Ortholog: 1p36.11Molecular Function: ATP binding; magnesium ion binding; MAP kinase kinase kinase activityBiological Process: activation of JNK activity; protein amino acid phosphorylation; signal transduction |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a serine/threonine protein kinase that forms a component of protein kinase-mediated signal transduction cascades. The encoded kinase participates in the regulation of vascular endothelial growth factor (VEGF) expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | O95382 |
| NCBI GenInfo Identifier: | 262527573 |
| NCBI Gene ID: | 9064 |
| NCBI Accession: | O95382.3 |
| UniProt Secondary Accession: | O95382,Q59HF4, Q5SSD4, Q75PK3, Q96B75, A2ACE8, A2VDG4 A2VDG5 |
| UniProt Related Accession: | O95382, JE0363 |
| Molecular Weight: | 141,648 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 6 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 6 |
| NCBI Official Symbol: | MAP3K6 |
| NCBI Official Synonym Symbols: | ASK2; MEKK6; MAPKKK6 |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 6 |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 6 |
| UniProt Synonym Protein Names: | Apoptosis signal-regulating kinase 2 |
| Protein Family: | Mitogen-activated protein kinase kinase kinase |
| UniProt Gene Name: | MAP3K6 |
| UniProt Entry Name: | M3K6_HUMAN |
| Antibodies |
| MAP3K6 Antibody (PACO05678) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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