Antibody Name:	Map2k5 Antibody (PACO21465)
Antibody SKU:	PACO21465
Size:	100ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:1000
Species Reactivity:	Human, Rat, Mouse
Immunogen:	Peptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.

Form:	Liquid
Storage Buffer:	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody.

Background:

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7)phosphorylates and activates the JNK signal transduction pathway. Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926. Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.

Synonyms:

MAP kinase kinase 5; Map2k5; MAPKK 5; MAPK/ERK kinase 5;

UniProt Protein Function:	MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 familyCellular Component: membrane; spindle; intracellular; nucleus; cytosolMolecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activityBiological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation
UniProt Protein Details:
NCBI Summary:	kinase involved in signal transduction in the cell [RGD, Feb 2006]
UniProt Code:	Q62862
NCBI GenInfo Identifier:	2499634
NCBI Gene ID:	29568
NCBI Accession:	Q62862.1
UniProt Secondary Accession:	Q62862,Q62863, Q62864
UniProt Related Accession:	Q62862
Molecular Weight:	448
NCBI Full Name:	Dual specificity mitogen-activated protein kinase kinase 5
NCBI Synonym Full Names:	mitogen activated protein kinase kinase 5
NCBI Official Symbol:	Map2k5
NCBI Official Synonym Symbols:	Mek5
NCBI Protein Information:	dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; MAP kinase kinase 5
UniProt Protein Name:	Dual specificity mitogen-activated protein kinase kinase 5
UniProt Synonym Protein Names:	MAPK/ERK kinase 5; MEK 5
Protein Family:	Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name:	Map2k5
UniProt Entry Name:	MP2K5_RAT