MAN2B2 Antibody (PACO56250)
- SKU:
- PACO56250
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MAN2B2 Antibody (PACO56250) |
| Antibody SKU: | PACO56250 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Epididymis-specific alpha-mannosidase protein (347-523AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: MAN2B2 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114, 108 kDa. Observed band size: 114 kDa. |
 | IHC image of PACO56250 diluted at 1:100 and staining in paraffin-embedded human thyroid tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: MAN2B2 antibody at 1.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114, 108 kDa. Observed band size: 135 kDa. |
| Background: | extracellular exosome, lysosomal lumen, alpha-mannosidase activity, mannan endo-1,6-alpha-mannosidase activity, mannose metabolic process, oligosaccharide catabolic process, protein deglycosylation |
| Synonyms: | Epididymis-specific alpha-mannosidase (EC 3.2.1.24) (Mannosidase α class 2B member 2), MAN2B2, KIAA0935 |
| UniProt Protein Function: | MAN2B2: Belongs to the glycosyl hydrolase 38 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.2.1.24; Secreted, signal peptide; Glycan Metabolism - other glycan degradation; Hydrolase; SecretedChromosomal Location of Human Ortholog: 4p16.1Molecular Function: zinc ion binding; carbohydrate binding; alpha-mannosidase activityBiological Process: mannose metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9Y2E5 |
| NCBI GenInfo Identifier: | 50659093 |
| NCBI Gene ID: | 23324 |
| NCBI Accession: | NP_056089.1 |
| UniProt Secondary Accession: | Q9Y2E5,Q66MP2, Q86T67 |
| UniProt Related Accession: | Q9Y2E5 |
| Molecular Weight: | 113,979 Da |
| NCBI Full Name: | epididymis-specific alpha-mannosidase isoform 1 |
| NCBI Synonym Full Names: | mannosidase, alpha, class 2B, member 2 |
| NCBI Official Symbol: | MAN2B2 |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | epididymis-specific alpha-mannosidase; core-specific lysosomal alpha-1,6-Mannosidase |
| UniProt Protein Name: | Epididymis-specific alpha-mannosidase |
| UniProt Synonym Protein Names: | Mannosidase alpha class 2B member 2 |
| Protein Family: | Epididymis-specific alpha-mannosidase |
| UniProt Gene Name: | MAN2B2 |
| UniProt Entry Name: | MA2B2_HUMAN |
| Antibodies |
| MAN2B2 Antibody (PACO10354) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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