Metabolism Assays
Maltose Assay Kit (Colorimetric or Fluorometric) (BA0132)
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- SKU:
- BA0132
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Detection Method:
- Fluorometric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Urine, Food, Beverages
- Research Area:
- TCA Cycle
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
Description
Maltose Assay Kit - Information
MALTOSE (C12H22O11) is a disaccharide, composed of two glucose units linked by an alpha bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products.
Assay Genie's maltose assay provides a simple, one step assay for measuring maltose. In this assay, maltose is converted to two glucoses, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at Ex/Em = 530/585 nm is directly proportional to maltose concentration in the sample.Applications
Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, etc.Maltose Assay Kit - Key Features
- Fast and sensitive. Use of 10 uL sample. Linear detection range 2 to 500 uM maltose in for colorimetric assays and 1 to 50 uM for flourimetric assays.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Maltose Assay Kit Information:
| Kit Includes: | Assay Buffer: 10 mL, Dye Reagent: 120 uL, Enzyme A: 120 uL, Standard: 1 mL, 5 mM Maltose Enzyme Mix: 120 uL |
| Kit Requires: | Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader |
| Method of Detection: | OD570nm, or FL530/585nm |
| Detection Limit: | 2 uM |
| Samples: | Serum, urine, food and beverages, etc. |
| Species: | All |
| Protocol Length: | 60 min |
| Size: | 100 tests |
| Storage: | Store all components at -20°C upon receiving |
| Shelf Life: | 6 months |
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