Metabolism Assays

Malate Assay Kit (BA0129)

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SKU:
BA0129
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Cells, Tissue, Malate In Food, Juice, Beverage, Other Agricultural Products
Research Area:
TCA Cycle
Research Area:
Urea
Research Area:
Diabetes & Obesity
Research Area:
Food Safety & Analysis
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry

Description

ELISA Kit Technical ManualMSDS

Malate Assay Kit

L-malate , or L-Malic acid, is a dicarboxylic acid that is made by all living organisms and plays an important role in the Calvin and Krebs Cycle. It is a source of CO2 for the Calvin cycle in plants and is also an intermediate that forms from fumarate in the Krebs Cycle. Malate is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, etc.

Assay Genie's L-malate assay kit is based on malate dehydrogenase catalyzed oxidation of malate in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm is proportional to the malate concentration in the sample.

Applications

For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism.

Key Features

  • Fast and sensitive. Use of 20 uL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Malate Assay Kit Information:

Method of Detection: OD565nm
Detection Limit: 0.02 mM
Samples: Malate in food, juice, beverage and other agricultural products.
Species: All
Protocol Length: 15 min
Size: 100 tests
Shelf Life: 6 months

 

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