Description
Antibody Name: | MAGED2 Antibody (PACO42458) |
Antibody SKU: | PACO42458 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC, IF, IP |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000, |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Melanoma-associated antigen D2 protein (38-285AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate. All lanes: MAGED2 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65, 64 kDa. Observed band size: 65, 64 kDa. |
![]() | IHC image of PACO42458 diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | IHC image of PACO42458 diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
Background: | membrane |
Synonyms: | Melanoma-associated antigen D2 (11B6) (Breast cancer-associated gene 1 protein) (BCG-1) (Hepatocellular carcinoma-associated protein JCL-1) (MAGE-D2 antigen), MAGED2, BCG1 |
UniProt Protein Function: | MAGE-D2: 2 isoforms of the human protein are produced by alternative splicing. |
UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: membrane |
NCBI Summary: | This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
UniProt Code: | Q9UNF1 |
NCBI GenInfo Identifier: | 17380153 |
NCBI Gene ID: | 10916 |
NCBI Accession: | Q9UNF1.2 |
UniProt Secondary Accession: | Q9UNF1,O76058, Q5BJF3, Q8NAL6, Q9H218, Q9P0U9, Q9UM52 A6NMX0, |
UniProt Related Accession: | Q9UNF1 |
Molecular Weight: | 606 |
NCBI Full Name: | Melanoma-associated antigen D2 |
NCBI Synonym Full Names: | melanoma antigen family D, 2 |
NCBI Official Symbol: | MAGED2 |
NCBI Official Synonym Symbols: | 11B6; BCG1; BCG-1; HCA10; MAGE-D2 |
NCBI Protein Information: | melanoma-associated antigen D2; MAGE-D2 antigen; breast cancer-associated gene 1 protein; hepatocellular carcinoma-associated protein JCL-1 |
UniProt Protein Name: | Melanoma-associated antigen D2 |
UniProt Synonym Protein Names: | 11B6; Breast cancer-associated gene 1 protein; BCG-1; Hepatocellular carcinoma-associated protein JCL-1; MAGE-D2 antigen |
UniProt Gene Name: | MAGED2 |
UniProt Entry Name: | MAGD2_HUMAN |
Antibodies |
MAGED2 Antibody (PACO10345) |
Secondary Antibody |
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
Recommended Products |
Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
Anti-HRP-conjugated Beta Actin Antibody (CABC028) |