MAGED2 Antibody (PACO42458)
- SKU:
- PACO42458
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Applications:
- IP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | MAGED2 Antibody (PACO42458) |
| Antibody SKU: | PACO42458 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000, |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Melanoma-associated antigen D2 protein (38-285AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC-3 whole cell lysate. All lanes: MAGED2 antibody at 4.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65, 64 kDa. Observed band size: 65, 64 kDa. |
 | IHC image of PACO42458 diluted at 1:320 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO42458 diluted at 1:320 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | membrane |
| Synonyms: | Melanoma-associated antigen D2 (11B6) (Breast cancer-associated gene 1 protein) (BCG-1) (Hepatocellular carcinoma-associated protein JCL-1) (MAGE-D2 antigen), MAGED2, BCG1 |
| UniProt Protein Function: | MAGE-D2: 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: membrane |
| NCBI Summary: | This gene is a member of the MAGED gene family. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR). This gene may also be involved in several types of cancer, including breast cancer and melanoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | Q9UNF1 |
| NCBI GenInfo Identifier: | 17380153 |
| NCBI Gene ID: | 10916 |
| NCBI Accession: | Q9UNF1.2 |
| UniProt Secondary Accession: | Q9UNF1,O76058, Q5BJF3, Q8NAL6, Q9H218, Q9P0U9, Q9UM52 A6NMX0, |
| UniProt Related Accession: | Q9UNF1 |
| Molecular Weight: | 606 |
| NCBI Full Name: | Melanoma-associated antigen D2 |
| NCBI Synonym Full Names: | melanoma antigen family D, 2 |
| NCBI Official Symbol: | MAGED2 |
| NCBI Official Synonym Symbols: | 11B6; BCG1; BCG-1; HCA10; MAGE-D2 |
| NCBI Protein Information: | melanoma-associated antigen D2; MAGE-D2 antigen; breast cancer-associated gene 1 protein; hepatocellular carcinoma-associated protein JCL-1 |
| UniProt Protein Name: | Melanoma-associated antigen D2 |
| UniProt Synonym Protein Names: | 11B6; Breast cancer-associated gene 1 protein; BCG-1; Hepatocellular carcinoma-associated protein JCL-1; MAGE-D2 antigen |
| UniProt Gene Name: | MAGED2 |
| UniProt Entry Name: | MAGD2_HUMAN |
| Antibodies |
| MAGED2 Antibody (PACO10345) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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