Name: LPAR6 Antibody (PACO63687)
SKU: PACO63687
Availability: OutOfStock

Antibody Name:	LPAR6 Antibody (PACO63687)
Antibody SKU:	PACO63687
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Peptide sequence from Human Lysophosphatidic acid, receptor 6 protein (292-313AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate. All lanes: LPAR6 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa.
	Immunofluorescence staining of HepG2 cells with PACO63687 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO63687 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Binds to oleoyl-L-alpha-lysophosphatidic acid, (LPA). Intracellular cAMP is involved in the receptor activation. Important for the maintenance of hair growth and texture.
Synonyms:	Lysophosphatidic acid, receptor 6 (LPA receptor 6) (LPA-6) (Oleoyl-L-alpha-lysophosphatidic acid, receptor) (P2Y purinoceptor 5) (P2Y5) (Purinergic receptor 5) (RB intron encoded G-protein coupled receptor), LPAR6, P2RY5

UniProt Protein Function:	P2RY5: Binds to oleoyl-L-alpha-lysophosphatidic acid (LPA). Intracellular cAMP is involved in the receptor activation. Important for the maintenance of hair growth and texture. Defects in LPAR6 are the cause of woolly hair autosomal recessive type 1 with or without hypotrichosis (ARWH1). A hair shaft disorder characterized by fine and tightly curled hair. Compared to normal curly hair that is observed in some populations, woolly hair grows slowly and stops growing after a few inches. Under light microscopy, woolly hair shows some structural anomalies, including trichorrhexis nodosa and tapered ends. Defects in LPAR6 are the cause of hypotrichosis type 8 (HYPT8). A condition characterized by the presence of less than the normal amount of hair. Affected individuals show progressive hair loss, thinning of scalp hair since early childhood, sparse body hair, and sparse eyebrows and eyelashes in some cases. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-passChromosomal Location of Human Ortholog: 13q14Cellular Component: plasma membrane; integral to membraneMolecular Function: G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathwayDisease: Hypotrichosis 8
UniProt Protein Details:
NCBI Summary:	The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene aligns with an internal intron of the retinoblastoma susceptibility gene in the reverse orientation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
UniProt Code:	P43657
NCBI GenInfo Identifier:	241982708
NCBI Gene ID:	10161
NCBI Accession:	NP_001155970.1
UniProt Secondary Accession:	P43657,O15133, Q3KPF5, Q53FA0, Q5VW44, Q7Z3S0, Q7Z3S6 A4FTW9, B3KVF2
UniProt Related Accession:	P43657
Molecular Weight:	39,392 Da
NCBI Full Name:	lysophosphatidic acid receptor 6
NCBI Synonym Full Names:	lysophosphatidic acid receptor 6
NCBI Official Symbol:	LPAR6
NCBI Official Synonym Symbols:	LAH3; P2Y5; ARWH1; P2RY5
NCBI Protein Information:	lysophosphatidic acid receptor 6; LPA-6; LPA receptor 6; P2Y purinoceptor 5; purinergic receptor 5; G-protein coupled purinergic receptor P2Y5; RB intron encoded G-protein coupled receptor; oleoyl-L-alpha-lysophosphatidic acid receptor; purinergic recepto
UniProt Protein Name:	Lysophosphatidic acid receptor 6
UniProt Synonym Protein Names:	Oleoyl-L-alpha-lysophosphatidic acid receptor; P2Y purinoceptor 5; P2Y5; Purinergic receptor 5; RB intron encoded G-protein coupled receptor
Protein Family:	Lysophosphatidic acid receptor
UniProt Gene Name:	LPAR6
UniProt Entry Name:	LPAR6_HUMAN