LMO7 Antibody (PACO60236)
- SKU:
- PACO60236
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | LMO7 Antibody (PACO60236) |
| Antibody SKU: | PACO60236 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human LIM domain only protein 7 protein (1301-1465AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60236 diluted at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO60236 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60236 diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | cytoplasm, cytosol, focal adhesion, nucleus, ubiquitin ligase complex, ubiquitin-protein transferase activity, post-translational protein modification, protein polyubiquitination, protein ubiquitination |
| Synonyms: | LIM domain only protein 7 (LMO-7) (F-box only protein 20) (LOMP), LMO7, FBX20 FBXO20 KIAA0858 |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein containing a calponin homology (CH) domain, a PDZ domain, and a LIM domain, and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, however, the full-length nature of some variants is not known. [provided by RefSeq, Jan 2009] |
| UniProt Code: | Q8WWI1 |
| NCBI GenInfo Identifier: | 308153585 |
| NCBI Gene ID: | 4008 |
| NCBI Accession: | Q8WWI1.3 |
| UniProt Secondary Accession: | Q8WWI1,O15462, O95346, Q5TBK6, Q9UKC1, Q9UQM5, Q9Y6A7 E9PLH4 |
| UniProt Related Accession: | Q8WWI1 |
| Molecular Weight: | 158,265 Da |
| NCBI Full Name: | LIM domain only protein 7 |
| NCBI Synonym Full Names: | LIM domain 7 |
| NCBI Official Symbol: | LMO7 |
| NCBI Official Synonym Symbols: | LOMP; FBX20; FBXO20 |
| NCBI Protein Information: | LIM domain only protein 7 |
| UniProt Protein Name: | LIM domain only protein 7 |
| UniProt Synonym Protein Names: | F-box only protein 20; LOMP |
| Protein Family: | LIM domain only protein |
| UniProt Gene Name: | LMO7 |
| UniProt Entry Name: | LMO7_HUMAN |
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