Antibody Name:	LMCD1 Antibody (PACO58520)
Antibody SKU:	PACO58520
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human LIM and cysteine-rich domains protein 1 protein (105-268AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of A549 cells with PACO58520 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO58520 diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter (By similarity). Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.

Synonyms:

LIM and cysteine-rich domains protein 1 (Dyxin), LMCD1

UniProt Protein Function:	LMCD1: Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue- specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.Protein type: Unknown functionChromosomal Location of Human Ortholog: 3p26-p24Cellular Component: extracellular matrix; extracellular space; cytoplasm; nucleusMolecular Function: zinc ion binding; transcription corepressor activityBiological Process: transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
UniProt Code:	Q9NZU5
NCBI GenInfo Identifier:	20978521
NCBI Gene ID:	29995
NCBI Accession:	Q9NZU5.1
UniProt Secondary Accession:	Q9NZU5,B4DG80
UniProt Related Accession:	Q9NZU5
Molecular Weight:	32,882 Da
NCBI Full Name:	LIM and cysteine-rich domains protein 1
NCBI Synonym Full Names:	LIM and cysteine-rich domains 1
NCBI Official Symbol:	LMCD1
NCBI Official Synonym Symbols:
NCBI Protein Information:	LIM and cysteine-rich domains protein 1; dyxin
UniProt Protein Name:	LIM and cysteine-rich domains protein 1
UniProt Synonym Protein Names:	Dyxin
Protein Family:	LIM and cysteine-rich domains protein
UniProt Gene Name:	LMCD1
UniProt Entry Name:	LMCD1_HUMAN