ligA Antibody (PACO34258)
- SKU:
- PACO34258
- Product Type:
- Antibody
- Reactivity:
- Bacteria - Escherichia coli
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | ligA Antibody (PACO34258) |
| Antibody SKU: | PACO34258 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Escherichia coli |
| Immunogen: | Recombinant Escherichia coli DNA ligase protein (1-671AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: DNA ligase antibody at 2µg/ml + DH5a whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 74 kDa. Observed band size: 74 kDa. |
| Background: | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
| Synonyms: | DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD(+)]), ligA, dnaL lig lop pdeC |
| UniProt Protein Function: | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
| UniProt Protein Details: | |
| NCBI Summary: | ligA is an essential gene. [More information is available at EcoGene: EG10534]. LigA is one of two known NAD(+)-dependent DNA ligases, catalyzing the formation of phosphodiester bonds in duplex DNA. [More information is available at EcoCyc: EG10534]. |
| UniProt Code: | P15042 |
| NCBI GenInfo Identifier: | 16130337 |
| NCBI Gene ID: | 946885 |
| NCBI Accession: | NP_416906.1 |
| UniProt Secondary Accession: | P15042,P78197, P78198 |
| UniProt Related Accession: | P15042 |
| Molecular Weight: | 73,606 Da |
| NCBI Full Name: | DNA ligase, NAD(+)-dependent |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | ligA |
| NCBI Official Synonym Symbols: | dnaL; ECK2406; JW2403; lig; lop; pdeC |
| NCBI Protein Information: | DNA ligase, NAD(+)-dependent |
| UniProt Protein Name: | DNA ligase |
| UniProt Synonym Protein Names: | Polydeoxyribonucleotide synthase [NAD(+)] |
| Protein Family: | DNA |
| UniProt Gene Name: | ligA |
| UniProt Entry Name: | DNLJ_ECOLI |
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