lexA Antibody (PACO50402)

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SKU:
PACO50402

Description

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Antibody Name:lexA Antibody (PACO50402)
Antibody SKU:PACO50402
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:Escherichia coli
Immunogen:Recombinant Escherichia coli LexA repressor protein (1-202AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in Recombinant protein. All lanes: lexA antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 39 kDa. observed band size: 39 kDa..
Background:Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.
Synonyms:LexA repressor (EC 3.4.21.88), lexA, exrA spr tsl umuA
UniProt Protein Function:Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
UniProt Protein Details:
NCBI Summary:LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533].
UniProt Code:P0A7C2
NCBI GenInfo Identifier:16131869
NCBI Gene ID:948544
NCBI Accession:NP_418467.1
UniProt Secondary Accession:P0A7C2,P03033, Q2M6R1
UniProt Related Accession:P0A7C2
Molecular Weight:38.4 kDa
NCBI Full Name:transcriptional repressor of SOS regulon
NCBI Synonym Full Names:
NCBI Official Symbol:lexA  
NCBI Official Synonym Symbols:ECK4035; exrA; JW4003; spr; tsl; umuA  
NCBI Protein Information:transcriptional repressor of SOS regulon
UniProt Protein Name:LexA repressor
UniProt Synonym Protein Names:
Protein Family:LexA repressor
UniProt Gene Name:lexA  
UniProt Entry Name:LEXA_ECOLI
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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Additional Information

Product Type:
Antibody
Reactivity:
Bacteria - Escherichia coli
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
Applications:
WB
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
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