Antibody Name:	lexA Antibody (PACO50402)
Antibody SKU:	PACO50402
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:	Escherichia coli
Immunogen:	Recombinant Escherichia coli LexA repressor protein (1-202AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in Recombinant protein. All lanes: lexA antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. predicted band size: 39 kDa. observed band size: 39 kDa..

Background:

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.

Synonyms:

LexA repressor (EC 3.4.21.88), lexA, exrA spr tsl umuA

UniProt Protein Function:	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment (PubMed:20005847).The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352).
UniProt Protein Details:
NCBI Summary:	LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533].
UniProt Code:	P0A7C2
NCBI GenInfo Identifier:	16131869
NCBI Gene ID:	948544
NCBI Accession:	NP_418467.1
UniProt Secondary Accession:	P0A7C2,P03033, Q2M6R1
UniProt Related Accession:	P0A7C2
Molecular Weight:	38.4 kDa
NCBI Full Name:	transcriptional repressor of SOS regulon
NCBI Synonym Full Names:
NCBI Official Symbol:	lexA
NCBI Official Synonym Symbols:	ECK4035; exrA; JW4003; spr; tsl; umuA
NCBI Protein Information:	transcriptional repressor of SOS regulon
UniProt Protein Name:	LexA repressor
UniProt Synonym Protein Names:
Protein Family:	LexA repressor
UniProt Gene Name:	lexA
UniProt Entry Name:	LEXA_ECOLI