LEMD1 Antibody (PACO61394)
- SKU:
- PACO61394
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | LEMD1 Antibody (PACO61394) |
| Antibody SKU: | PACO61394 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human LEM domain-containing protein 1 protein (44-151AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO61394 diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO61394 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Synonyms: | LEM domain-containing protein 1, Cancer/testis antigen 50, CT50, LEM domain protein 1, LEMP-1, LEMD1 |
| UniProt Protein Function: | LEMD1: 6 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, integral; Cancer Testis Antigen (CTA)Chromosomal Location of Human Ortholog: 1q32.1Cellular Component: integral to membrane |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q68G75 |
| NCBI GenInfo Identifier: | 312261248 |
| NCBI Gene ID: | 93273 |
| NCBI Accession: | NP_001185979.1 |
| UniProt Secondary Accession: | Q68G75,Q6L9T9, Q6L9U0, Q6L9U1, Q6L9U2, Q6L9U3, Q6L9U4 |
| UniProt Related Accession: | Q68G75 |
| Molecular Weight: | 7,503 Da |
| NCBI Full Name: | LEM domain-containing protein 1 isoform 1 |
| NCBI Synonym Full Names: | LEM domain containing 1 |
| NCBI Official Symbol: | LEMD1 |
| NCBI Official Synonym Symbols: | CT50; LEMP-1 |
| NCBI Protein Information: | LEM domain-containing protein 1; LEM domain protein 1; cancer/testis antigen 50 |
| UniProt Protein Name: | LEM domain-containing protein 1 |
| UniProt Synonym Protein Names: | Cancer/testis antigen 50; CT50; LEM domain protein 1; LEMP-1 |
| Protein Family: | LEM domain-containing protein |
| UniProt Gene Name: | LEMD1 |
| UniProt Entry Name: | LEMD1_HUMAN |
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