Enzymes Recombinant Proteins

LDHB Recombinant Protein (RPPB1898)

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SKU:
RPPB1898
Product Type:
Recombinant Protein
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:LDHB Recombinant Protein
Product Code:RPPB1898
Size:3mg
Target:LDHB
Synonyms:Lactate Dehydrogenase, LDH.
Source:Escherichia Coli
Physical Appearance:Sterile lyophilized powder.
Formulation:The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.
Solubility:It is recommended to reconstitute the lyophilized LDH in sterile 18M?-cm H2O not less than 100 �g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity:The specific activity was found to be 258 U/mg protein.

Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.

The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart.

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system_update_altDatasheetHuman LDH-B/LDHB Recombinant Protein L-Lactate Dehydrogenase B Chain (LDH-B) is a member of the lactate dehydrogenase family that consists of three members, LDH-A, LDH-B