Name: LAYN Antibody (PACO18143)
SKU: PACO18143
Availability: OutOfStock

Antibody Name:	LAYN Antibody (PACO18143)
Antibody SKU:	PACO18143
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:	Human, Mouse
Immunogen:	Synthetic peptide of human LAYN

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue, Primary antibody: PACO18143(LAYN Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.

Background:

Layilin is a recently cloned ˜55-kDa membrane-binding partner for talin (Borowsky and Hynes, 1998 ) that is widely expressed in different cell types and tissue extracts. It is found in peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells induced to migrate in in vitro wounding experiments. Layilin colocalizes with talin in ruffles and binds to talin's ˜50-kDa head domain (amino acid, 280435). Other parts of talin (the ˜220-kDa tail fragment) can bind β -integrin cytoplasmic tails, vinculin, and F-actin and hence form an integrin-cytoskeleton linkage at sites of cell-substratum contact. Layilin's extracellular domain is homologous with the carbohydrate-recognition domains (CRD) of C-type lectins.

Synonyms:

layilin

UniProt Protein Function:	LAYN: Receptor for hyaluronate. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23.1 Cellular Component: ruffle; cell surface; focal adhesion; integral to membrane Molecular Function:hyaluronic acid binding; carbohydrate binding
UniProt Code:	Q6UX15
NCBI GenInfo Identifier:	74738165
NCBI Gene ID:	143903
NCBI Accession:	Q6UX15.1
UniProt Secondary Accession:	Q6UX15,Q8TAY8, Q96NC5, Q96NF3, A6NJB0, B4DJU0,
UniProt Related Accession:	Q6UX15
Molecular Weight:	382
NCBI Full Name:	Layilin
NCBI Synonym Full Names:	layilin
NCBI Official Symbol:	LAYN
NCBI Protein Information:	layilin
UniProt Protein Name:	Layilin
Protein Family:	Layilin
UniProt Gene Name:	LAYN
UniProt Entry Name:	LAYN_HUMAN