L-Lactic Acid/Lactate Assay Kit - Colorimetric (MAES0056)
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- SKU:
- MAES0056
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Cell Culture Supernatant, Tissue, Cells
- Sensitivity:
- 0.10 mmol/L
- Range:
- 0.12-7.0 mmol/L
- Assay Time:
- 30 min
- Shipping:
- Gel Pack
- Research Area:
- Food Safety & Analysis
- Research Area:
- Diabetes & Obesity
- Research Area:
- Glycolysis & Carbohydrates
Description
system_update_altDatasheet
| Product Name: | L-Lactic Acid/Lactate Assay Kit - Colorimetric |
| Product Code: | MAES0056 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, cell culture supernatant, tissue, cells |
| Assay Time: | 30 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.10 mmol/L |
| Detection Range: | 0.12-7.0 mmol/L |
| Recovery Rate: | 105 |
| Inter CV: | 3.5 |
| Inter CV: | 1.4 |
Lactic acid is an intermediate product of glucose metabolism in the body, which is mainly produced by red blood cells, striated muscle and brain tissue. The concentration of lactic acid in the blood mainly depends on the synthesis speed and metabolic rate of liver and kidney. The bidirectional conversion of lactic acid and pyruvate is regulated by lactate dehydrogenase (LDH).
Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Glycolysis & Carbohydrates |
| Neto et al. | 2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identified | bioRxiv 2023 | View Citation |
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