KY Antibody (PACO54318)
- SKU:
- PACO54318
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | KY Antibody (PACO54318) |
| Antibody SKU: | PACO54318 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Kyphoscoliosis peptidase protein (23-170AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, U87 whole cell lysate. All lanes: KY antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64, 42, 73, 76 kDa. Observed band size: 64 kDa. |
 | Immunofluorescence staining of Hela cells with PACO54318 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO54318 at dilution of 1:100. |
| Background: | Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC (By similarity). |
| Synonyms: | Kyphoscoliosis peptidase (EC 3.4), KY |
| UniProt Protein Function: | KY: Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC. Belongs to the transglutaminase-like superfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiation; EC 3.4.-.-; Cell adhesion; ProteaseChromosomal Location of Human Ortholog: 3q22.2Cellular Component: cytoskeleton; Z discMolecular Function: peptidase activityBiological Process: muscle development; proteolysis; neuromuscular junction development |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8NBH2 |
| NCBI GenInfo Identifier: | 148806932 |
| NCBI Gene ID: | 339855 |
| NCBI Accession: | NP_848649.3 |
| UniProt Secondary Accession: | Q8NBH2,Q6ZT15, B7Z1S4 |
| UniProt Related Accession: | Q8NBH2 |
| Molecular Weight: | 63855 |
| NCBI Full Name: | kyphoscoliosis peptidase |
| NCBI Synonym Full Names: | kyphoscoliosis peptidase |
| NCBI Official Symbol: | KY |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | kyphoscoliosis peptidase |
| UniProt Protein Name: | Kyphoscoliosis peptidase |
| UniProt Synonym Protein Names: | |
| Protein Family: | Kynureninase |
| UniProt Gene Name: | KY |
| UniProt Entry Name: | KY_HUMAN |
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