KRT76 Antibody (PACO60749)
- SKU:
- PACO60749
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | KRT76 Antibody (PACO60749) |
| Antibody SKU: | PACO60749 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Keratin, type II cytoskeletal 2 oral protein (173-283AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: KRT76 antibody at 4.9µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 66 kDa. Observed band size: 66 kDa. |
 | Immunofluorescence staining of SH-SY5Y cells with PACO60749 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO60749 diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Probably contributes to terminal cornification. |
| Synonyms: | Keratin, type II cytoskeletal 2 oral (Cytokeratin-2P) (CK-2P) (K2P) (Keratin-76) (K76) (Type-II keratin Kb9), KRT76, KRT2B KRT2P |
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q01546 |
| NCBI GenInfo Identifier: | 47132620 |
| NCBI Gene ID: | 3849 |
| NCBI Accession: | NP_000414.2 |
| UniProt Secondary Accession: | Q01546,Q01546, P12035, P13647, P02538, P04259, P48668 Q3SY84, Q14CN4, Q86Y46 |
| UniProt Related Accession: | Q01546,P35908 |
| Molecular Weight: | 65,433 Da |
| NCBI Full Name: | keratin, type II cytoskeletal 2 epidermal |
| NCBI Synonym Full Names: | keratin 2, type II |
| NCBI Official Symbol: | KRT2 |
| NCBI Official Synonym Symbols: | K2e; KRTE; CK-2e; KRT2A; KRT2E |
| NCBI Protein Information: | keratin, type II cytoskeletal 2 epidermal; cytokeratin-2e; epithelial keratin-2e; keratin-2 epidermis; type-II keratin Kb2 |
| UniProt Protein Name: | Keratin, type II cytoskeletal 2 epidermal |
| UniProt Synonym Protein Names: | Cytokeratin-2e; CK-2e; Epithelial keratin-2e; Keratin-2 epidermis; Keratin-2e; K2e; Type-II keratin Kb2 |
| Protein Family: | |
| UniProt Gene Name: | KRT2 |
| UniProt Entry Name: | K22E_HUMAN |
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