Name: KLHL12 Antibody (PACO36206)
SKU: PACO36206
Availability: OutOfStock

Antibody Name:	KLHL12 Antibody (PACO36206)
Antibody SKU:	PACO36206
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Kelch-like protein 12 protein (1-220AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO36206 diluted at 1:800 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of PC-3 cells with PACO36206 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.

Synonyms:

Kelch-like protein 12 (CUL3-interacting protein 1) (DKIR homolog) (hDKIR), KLHL12, C3IP1

UniProt Protein Function:	KLHL12: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q32.1 Cellular Component: Golgi membrane; ER to Golgi transport vesicle; cytosol Molecular Function:protein binding Biological Process: COPII coating of Golgi vesicle; protein monoubiquitination; ER to Golgi vesicle-mediated transport; Wnt receptor signaling pathway
NCBI Summary:	This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:	Q53G59
NCBI GenInfo Identifier:	97054498
NCBI Gene ID:	59349
NCBI Accession:	Q53G59.2
UniProt Related Accession:	Q53G59
Molecular Weight:	63kDa
NCBI Full Name:	Kelch-like protein 12
NCBI Synonym Full Names:	kelch like family member 12
NCBI Official Symbol:	KLHL12
NCBI Official Synonym Symbols:	DKIR; C3IP1
NCBI Protein Information:	kelch-like protein 12
UniProt Protein Name:	Kelch-like protein 12
UniProt Synonym Protein Names:	CUL3-interacting protein 1; DKIR homolog; hDKIR
Protein Family:	Kelch-like protein
UniProt Gene Name:	KLHL12
UniProt Entry Name:	KLH12_HUMAN