Product Name:	KLF Transcription Factor Activity Assay
Product Code:	TFAB00193
Target:	KLF
Synonyms:	Erythroid krueppel-like transcription factor, EKLF
Reactivity:	Human, Mouse, Rat
Sample Types:	Nuclear or cell lysates

The Assay Genie KLF transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	KLF1: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4). It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin. Belongs to the krueppel C2H2-type zinc-finger protein family.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19p13.13 Cellular Component: nuclear chromatin; nucleus Molecular Function:transcription factor activity Biological Process: erythrocyte differentiation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent Disease: Anemia, Congenital Dyserythropoietic, Type Iv; Blood Group--lutheran Inhibitor; Fetal Hemoglobin Quantitative Trait Locus 6
NCBI Summary:	This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009]
UniProt Code:	Q13351
NCBI GenInfo Identifier:	2501699
NCBI Gene ID:	10661
NCBI Accession:	Q13351.1
UniProt Secondary Accession:	Q13351,Q6PIJ5, Q92899,
UniProt Related Accession:	Q13351
Molecular Weight:	38kDa
NCBI Full Name:	Krueppel-like factor 1
NCBI Synonym Full Names:	Kruppel like factor 1
NCBI Official Symbol:	KLF1
NCBI Official Synonym Symbols:	EKLF
NCBI Protein Information:	Krueppel-like factor 1
UniProt Protein Name:	Krueppel-like factor 1
UniProt Synonym Protein Names:	Erythroid krueppel-like transcription factor; EKLF
Protein Family:	Krueppel-like factor
UniProt Gene Name:	KLF1