KIF3C Antibody (PACO59764)
- SKU:
- PACO59764
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | KIF3C Antibody (PACO59764) |
| Antibody SKU: | PACO59764 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Kinesin-like protein KIF3C protein (158-267AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate. All lanes: KIF3C antibody at 4.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 kDa. Observed band size: 90 kDa. |
 | Immunofluorescence staining of A549 cells with PACO59764 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59764 diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Microtubule-based anterograde translocator for membranous organelles. |
| Synonyms: | Kinesin-like protein KIF3C, KIF3C |
| UniProt Protein Function: | KIF3C: Microtubule-based anterograde translocator for membranous organelles. Belongs to the kinesin-like protein family. Kinesin II subfamily.Protein type: Microtubule-binding; MotorChromosomal Location of Human Ortholog: 2p23Cellular Component: cytosol; kinesin complexMolecular Function: ATPase activity; microtubule motor activity; motor activityBiological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule-based movement; retrograde vesicle-mediated transport, Golgi to ER |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | O14782 |
| NCBI GenInfo Identifier: | 308153589 |
| NCBI Gene ID: | 3797 |
| NCBI Accession: | O14782.3 |
| UniProt Secondary Accession: | O14782,O43544, Q4ZG18, Q53SX5, Q562F7 |
| UniProt Related Accession: | O14782 |
| Molecular Weight: | 89,426 Da |
| NCBI Full Name: | Kinesin-like protein KIF3C |
| NCBI Synonym Full Names: | kinesin family member 3C |
| NCBI Official Symbol: | KIF3C |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | kinesin-like protein KIF3C |
| UniProt Protein Name: | Kinesin-like protein KIF3C |
| UniProt Synonym Protein Names: | |
| Protein Family: | Kinesin-like protein |
| UniProt Gene Name: | KIF3C |
| UniProt Entry Name: | KIF3C_HUMAN |
| Antibodies |
| KIF3C Antibody (PACO10104) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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