Antibody Name:	KIDINS220 Antibody (PACO51670)
Antibody SKU:	PACO51670
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Kinase D-interacting substrate of 220 kDa protein (1077-1370AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate. All lanes: KIDINS220 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 197, 186, 116, 195, 60 kDa. Observed band size: 197 kDa..
	Immunohistochemistry of paraffin-embedded human appendix tissue using PACO51670 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human colon cancer using PACO51670 at dilution of 1:100.

Background:

Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.

Synonyms:

Kinase D-interacting substrate of 220 kDa (Ankyrin repeat-rich membrane-spanning protein), KIDINS220, ARMS KIAA1250

UniProt Protein Function:	KIDINS220: Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin- mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration. Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 2p24 Cellular Component: nucleoplasm; protein complex; membrane; late endosome; integral to membrane; cytosol Molecular Function:PDZ domain binding Biological Process: activation of MAPKK activity; nerve growth factor receptor signaling pathway; in utero embryonic development; dendrite morphogenesis; cytoplasmic transport
UniProt Code:	Q9ULH0
NCBI GenInfo Identifier:	172044825
NCBI Gene ID:	57498
NCBI Accession:	Q9ULH0.3
UniProt Secondary Accession:	Q9ULH0,Q4VC08, Q6MZU2, Q9H889, Q9H9E4, Q9NT37, Q9UF42 A1L4N4,
UniProt Related Accession:	Q9ULH0
Molecular Weight:	1771
NCBI Full Name:	Kinase D-interacting substrate of 220 kDa
NCBI Synonym Full Names:	kinase D-interacting substrate, 220kDa
NCBI Official Symbol:	KIDINS220
NCBI Official Synonym Symbols:	ARMS
NCBI Protein Information:	kinase D-interacting substrate of 220 kDa; truncated KIDINS220; ankyrin repeat-rich membrane spanning; ankyrin repeat-rich membrane-spanning protein
UniProt Protein Name:	Kinase D-interacting substrate of 220 kDa
UniProt Synonym Protein Names:	Ankyrin repeat-rich membrane-spanning protein
Protein Family:	Kinase D-interacting substrate
UniProt Gene Name:	KIDINS220
UniProt Entry Name:	KDIS_HUMAN