α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit
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- SKU:
- MAES0236
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Animal And Plant Tissue
- Sensitivity:
- 8.3 U/L
- Range:
- 8.3-42.3 U/L
- Assay Time:
- 40 min
Description
| Product Name: | α-Ketoglutarate Dehydrogenase(α-KGDH)Activity Assay Kit |
| Product Code: | MAES0236 |
| Sample Type: | Serum, Plasma, Animal And Plant Tissue |
| Assay Time: | 40 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | α-KGDH |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 8.3 U/L |
| Detection Range: | 8.3-42.3 U/L |
| Inter-assay CV: | 0.085 |
| Intra-assay CV: | 0.015 |
α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Incubator, Centrifuge |
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