Name: JMY Antibody (PACO56078)
SKU: PACO56078
Availability: OutOfStock

Antibody Name:	JMY Antibody (PACO56078)
Antibody SKU:	PACO56078
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Junction-mediating and -regulatory protein (708-848AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO56078 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO56078 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

Synonyms:

Junction-mediating and -regulatory protein, JMY

UniProt Protein Function:	JMY: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. Belongs to the JMY family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: leading edge; nucleoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity Biological Process: cell cycle arrest; positive regulation of apoptosis; positive regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter
UniProt Code:	Q8N9B5
NCBI GenInfo Identifier:	172045777
NCBI Gene ID:	133746
NCBI Accession:	Q8N9B5.2
UniProt Secondary Accession:	Q8N9B5,A1L4P5, B5MDS2, B5MDT0,
UniProt Related Accession:	Q8N9B5
Molecular Weight:	110,280 Da
NCBI Full Name:	Junction-mediating and -regulatory protein
NCBI Synonym Full Names:	junction mediating and regulatory protein, p53 cofactor
NCBI Official Symbol:	JMY
NCBI Official Synonym Symbols:	WHAMM2; WHDC1L3
NCBI Protein Information:	junction-mediating and -regulatory protein
UniProt Protein Name:	Junction-mediating and -regulatory protein
Protein Family:	Junction-mediating and -regulatory protein
UniProt Gene Name:	JMY
UniProt Entry Name:	JMY_HUMAN