Metabolism Assays
Isocitrate Assay Kit (Colorimetric) (BA0089)
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- SKU:
- BA0089
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Food, Beverage, Cells, Tissue
- Research Area:
- Ferroptosis
- Research Area:
- TCA Cycle
- Research Area:
- Lipid Metabolism
- Research Area:
- Diabetes & Obesity
- Research Area:
- Food Safety & Analysis
- Research Area:
- Plant & Environmental Stress
Description
Isocitrate Assay Kit - Information
Assay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the isocitrate concentration.Applications
For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.Isocitrate Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 20 to 5000 for 10 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Isocitrate Assay Kit - Data Sheet | |
| Method of Detection | OD565nm |
| Detection Limit | 20 |
| Samples | Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.) |
| Species | All |
| Protocol Length | 10 min |
| Size | 100 tests |
| Shelf Life | 6 months |
More Details
ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing alpha-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.Related Products
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