IRX4 Antibody (PACO54314)
- SKU:
- PACO54314
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | IRX4 Antibody (PACO54314) |
| Antibody SKU: | PACO54314 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Iroquois-class homeodomain protein IRX-4 protein (287-363AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Colo320 whole cell lysate. All lanes: IRX4 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 58 kDa. Observed band size: 55 kDa. |
 | Immunofluorescence staining of MCF-7 cells with PACO54314 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Likely to be an important mediator of ventricular differentiation during cardiac development. |
| Synonyms: | Iroquois-class homeodomain protein IRX-4 (Homeodomain protein IRXA3) (Iroquois homeobox protein 4), IRX4, IRXA3 |
| UniProt Protein Function: | Likely to be an important mediator of ventricular differentiation during cardiac development. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | P78413 |
| NCBI GenInfo Identifier: | 520975438 |
| NCBI Gene ID: | 50805 |
| NCBI Accession: | NP_001265561.1 |
| UniProt Secondary Accession: | P78413,Q2NL64, Q9UHR2, B2RMW5, D3DTC5, H1AFL0, H1AFL1 |
| UniProt Related Accession: | P78413 |
| Molecular Weight: | 57,050 Da |
| NCBI Full Name: | iroquois-class homeodomain protein IRX-4 isoform b |
| NCBI Synonym Full Names: | iroquois homeobox 4 |
| NCBI Official Symbol: | IRX4 |
| NCBI Official Synonym Symbols: | IRXA3 |
| NCBI Protein Information: | iroquois-class homeodomain protein IRX-4 |
| UniProt Protein Name: | Iroquois-class homeodomain protein IRX-4 |
| UniProt Synonym Protein Names: | Homeodomain protein IRXA3; Iroquois homeobox protein 4 |
| Protein Family: | Iroquois-class homeodomain protein |
| UniProt Gene Name: | IRX4 |
| UniProt Entry Name: |
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