INPP5F Antibody (PACO62647)
- SKU:
- PACO62647
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | INPP5F Antibody (PACO62647) |
| Antibody SKU: | PACO62647 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence from Human Phosphatidylinositide phosphatase SAC2 protein (187-204AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of SH-SY5Y cells with PACO62647 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling. Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms. Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation. Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus. Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (By similarity). May play a role as negative regulator of axon regeneration after central nervous system injuries (By similarity). |
| Synonyms: | Phosphatidylinositide phosphatase SAC2 (EC 3.1.3.25) (Inositol polyphosphate 5-phosphatase F) (Sac domain-containing inositol phosphatase 2) (Sac domain-containing phosphoinositide 4-phosphatase 2) (hSAC2), INPP5F, KIAA0966 SAC2 |
| UniProt Protein Function: | INPP5F: Inositol 5-phosphatase which acts on phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Hydrolyzes phosphatidylinositol 4,5- bisphosphate most effectively. Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Phosphatase, lipid; EC 3.1.3.-Chromosomal Location of Human Ortholog: 10q26.11Cellular Component: clathrin-coated endocytic vesicle; coated pit; early endosome; early endosome membrane; recycling endosomeMolecular Function: protein binding; protein homodimerization activityBiological Process: cardiac muscle hypertrophy; negative regulation of axon regeneration; negative regulation of tyrosine phosphorylation of Stat3 protein; phosphatidylinositol biosynthetic process; phosphatidylinositol catabolic process; phosphoinositide dephosphorylation; phosphoinositide-mediated signaling; positive regulation of receptor recycling |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9Y2H2 |
| NCBI GenInfo Identifier: | 187611527 |
| NCBI Gene ID: | 22876 |
| NCBI Accession: | Q9Y2H2.3 |
| UniProt Secondary Accession: | Q9Y2H2,Q2T9J4, Q5W135, Q5W136, Q6NVY2, Q86U97, Q9H3D9 Q9NT51, B3KRF1, D3DRD1 |
| UniProt Related Accession: | Q9Y2H2 |
| Molecular Weight: | 57,800 Da |
| NCBI Full Name: | Phosphatidylinositide phosphatase SAC2 |
| NCBI Synonym Full Names: | inositol polyphosphate-5-phosphatase F |
| NCBI Official Symbol: | INPP5F |
| NCBI Official Synonym Symbols: | SAC2; hSAC2; MSTP007; MSTPO47 |
| NCBI Protein Information: | phosphatidylinositide phosphatase SAC2 |
| UniProt Protein Name: | Phosphatidylinositide phosphatase SAC2 |
| UniProt Synonym Protein Names: | Inositol polyphosphate 5-phosphatase F |
| Protein Family: | Phosphatidylinositide phosphatase |
| UniProt Gene Name: | INPP5F |
| UniProt Entry Name: | SAC2_HUMAN |
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