Cell Biology Assays

Indole Assay Kit (Colorimetric) (BA0046)

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SKU:: BA0046
UPC:
MPN:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Bacterial Growth Medium
Research Area:: Plant & Environmental Stress
Research Area:: High-Throughput Screening

Description
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Description

Indole Assay Kit - Information

Assay Genie's indole assay kit is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly proportional to the indole in the sample.

Applications

Indole determination in biological samples.

Indole Assay Kit - Key Features

Fast and sensitive. Use of 100uL sample. Linear detection range from 3 to 100 uM indole in 96-well plate assay.
Convenient. The procedure involves adding a single working reagent, and reading the absorbance immediately.

Indole Assay Kit - Data Sheet
Kit Includes	Reagent: 12 mL Standard: 100 uL (10 mM Indole)
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader
Method of Detection	OD565nm
Detection Limit	3 uM
Samples	Bacterial growth medium
Species	All
Protocol Length	Under 5 min
Size	100 tests
Storage	Store all components at 4°C upon receiving
Shelf Life	6 months

More Details

INDOLE is the primary product of tryptophan breakdown by tryptophanase. The indole test is commonly performed on bacteria to classify them on their ability to break down tryptophan to indole.